ࡱ>   !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~Root Entry\ob$OSL5ܿ@NativeContent_MAIN&cSummaryInformation(0Trial will consist of 4 dietary treatments SEach treatment will be fed to four randomly-assigned replicate aquaria for 10 weeksAquaria will be 20 gallons in size, set up as a recirculating system and maintained at a temperature of 27-29 C with all other water quality parameters in optimal ranges for juvenile Tilapiah15 juvenile Tilapia with an average weight of approximately 5 g each will be used per replicate aquaria 2 weeks prior to beginning the feeding trial, Tilapia in each aquaria willQPW9 5 wuguan1DAVISDA Arialr       NormalCommaCurrencyPercentFixedDate Heading 1 Heading 2 Total Comma0 Currency0 !PbPP"P"P PPPP`PPPPPP"P!P!P"P#P"PP @P!!P"PP"PP#P#P@P  @P! P"@P!!P"P @P @P!!P"P @P PPP@P !P"!P"!P"@P@P P!P @P! @P! @P!PP!P !P P!P P!P P!P P!P P$!P$ P!P P!P P"!P" P!P P!P P&!P& P(!P( P!P P!P !P P!P "P""P%PP!PP!PPPPP!P"PPPP"P@P!PPP "P!P""P"P!P"P"P"PP "PP !P"!P""P""P""P""P""PP "PP "P"PP P !P""P"PP!P"!P" P P @P @P @P"P P!P" @P# @P"!P*!P*!P*!P!*!P!*!P'*!P(*!P&*!P*!P*!P*!P*!P*!P*!P*PPP0PPPPP @P!!P"!P"@P!P PPPPP @P!@P!P P @P!P @P!P P !P"!P"!P"!P"PPPPPPPP$"P"PP P P P P P "P %P""P P P P P P P "P %P""P %P"PPPP$P"PP$P"PP$P"PfG?e;L0 gebDateacTrt #Tank #BiomassNo. FishMeanNo Fish% feedExperiment CodeMaterials and Methods`pHSpecific analyze#+ be fed Treatment 2 at a set percentage of body weight (based on fish size). At the end of conditioning period, the fish in each tank will be weighed, and each aquaria will be switched to its respective experimental diet. Fish will be fed to apparent satiation (on a dry weight feed basis) by feeding at a set percentage of body weight. Feed will be divided into two feedings per day.ZAny mortalities should be weighed and recorded daily then feed inputs adjusted accordingly6Fish will be weighed and feed amounts adjusted weekly This example weekly weights`At the end of the ten-week feeding period the total weight of Tilapia in each tank will be takenVData to be reported will include weight gain, total feed consumption, FE and survival General NitriteTAN7General growth trial (fish are weighed every 1-2 weeks) Dry matter% gainFCE Feed/fishWeeklyTrtWeightAverageFinal wt Pan + samplePanDietExp03-Cargil TilapiaMoisture content of feedsRoom 1KNote: I generally put the important things at the top, followed by methods.4K ArialSalinityTemp DOSystemWeek 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8LA Hobo, can be used for temperature but there is not garanttee of it workingWeek 9Week 10Final Data Summary1Daily and weekly data should be transfered here. Week 0Initial Mean WtTotal feed/fishFinal Mean Weight Survival (%) TreatmentTankDGrowth or other measures should be plotted to give visual of resultsKSee specific methods for biochemical analyses. Data should be entered here.NameMethods summary4Tilapia obtained from station stocks (date of spawn)"Single spawn, sex reversed with MT9Conditioned fish on diet 2 starting (Date) for two weeks Objective:Starting date of experiment Dry feed0Daily rations for each fish on a wet feed basis. Dry weighttank$Protein analyse (whole body example) sample (mg)HCL(ml)Protein CVTube + oil (g)Tube (g) Sample (g)ID Wet weight/Note: lipids are normally done on a wet sample.Final data means Lipids (%)oil% lipidsEnding date of the experimentTotal number of days or weeks-$    )   d )   d 2 P  d /Q /Q 5   d  .d d    Gz?       5   d  $   d  5   d  2 P  d /Q  3 ףp= ? d   P   d  d q P [k{:&$,@ {Gz? @ d  QX /Q 88d      jB d    B   `  `  ` %  c c  $  c c c        `  `  ` ,y ,y R1 x!! !<q!*Q!?!!R(!! 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" 'Q = ] @x R  FloatComment1 K"q!*yQ!?A!1! q#g ArialNew Romanxr#zvNote: for the first week the overal mean can be used. There after each tanks average weight (or biomass) must be used.t#u# "  " 'Q = 8 o @sary R  FloatComment2 |"q!*xQ!?A!1! q#g ArialNew Romanxr#To start an experiment the fish should be uniform in size and the biomasses similar. If there is to much variation (size or biomass) the experiment is a waste of time.t#u# "  " 'Q =  x C@ R  FloatComment4 W"q!*cQ!?A!1! q#g xArialNew Romansr#Weekly data is looked at in terms of weight gain, FCE etc. You must look at the data to make sure feed inputs and growth is good. t#u# "  " 'Q = x o @ R  FloatComment5 t"q!*cQ!?A!1! q#g ArialNew RomanEsEr#This should be at or near satiation. If FE is a critical measure, it should be slightly bellow satitation. Check for left over food 5-10 minutes after feeding.t#u# "  " 'Q = ? @ R  FloatComment3 "q!*2Q!?A!1! q#g ArialNew Roman#dr#If a fish dies then the feed input must be shifted. Durring the week we do this on the feed sheet manually, then start the new week with the right number. This makes it easier to calculate feed inputs.t#u# "  " 'Q =  x CHu mu R  FloatComment6 T"q!*Q!?A!1! q#g ArialNew Romanr#Note, if you do your means on the treatment you can check the formula! Then you can copy the formula to get the means you want.t#u# "   " 'Q = @ R  FloatComment7  "q!*QQ!?A!1! q#g ArialNew Roman$r#IETreatments should be randomly assigned for this example they are not!t#u# "  !" 'Q =t{x] @ R  FloatComment8 F""q!*yQ!?A!1! q#g ArialNew Roman1qr#uqIt was noticed the fish were not eating all the feed. Hence the ration was reduced to 4.5% (changed the formula) t#u# "t  #" 'Q =' @ R  FloatComment9 #$"q!*eqQ!?A!1! q#g ArialNew Romanqr#RNAgain, feed was left so the ration was reduced to 3% for the rest of the week.t#u# "      A   G!cdsdsd s 1l1l1!l!1(l(1/l/16l61=l= *AIAb     V   ccmcmcmcmcmcmcmcmcmcm |AHA?@@@       r ceeeeneeeeneeeeneeeeneeeeneeeeneeeeneeeeneeeeneeeen aAGUUUUUUW@Y@Y@Y@Y@UUUUUUW@UUUUUUW@Y@UUUUUUW@Y@Z@Y@Y@UUUUUUW@U@Y@A*X@*X@*X@Y@ u      z chff@أ@ff@t@9@ģ@أ@8@fft@`@L@t`@D@ @fffffC@43333D@@fffffC@ffffffB@fffff&D@@YD@E@B@D@°@J@o+DN@M@@?ܹ@7Dʲ@z@@B8?Dn@@N@@oظ@D @2@ @@@p@@&@@R@D)@@@@fffffQ@@o@B` ^@D.@/@B`~fffffa@D@%@]@e@e$@offfffa@@xyd@D m@@K@_@@@@+@e@0f@V@@oh@p@a@D@(p@Q@@@s@@`@q@DC@5@ c@X@oHq@Q"@fffffr@ZDg@'@K@t@fffff6c@D@E@L@@fffff>s@oD A<@N@@n@4@@!@]@@id@Dw@AQ@@@@o@@DS@1@A@.@@A@A@@fffff@D@@I@@o%@ AD d@ e@d@d@d@@d@d@@e@ e@ e@d@d@d@d@d@d@vyQ??{Gz?zGz?     c    # PccmboBb BoB oB oB oB oB oB oB o hAF@X@D@ 3@@ܓ@8@@@l@@@@8@= ףp}2@B@@ DQG@-@ ףp=A@D@       h    c gi6?gK~?g?g?g?gQ?g(\?g333333?gb/?gK~?g> ףp=?g?g(\?gt@ t?gY%X?pt@ t?g(\@gGzG@g(\@gGz@g ףp= @g(\@g:m@g~K~@gK~@gN@g333333@gףp= @g6i6@gzG@gBA@p@g%I$I@g@gt@ @g@g t@@gUUUUUU @g t@ @g:m@gj6i@g @g@gK~ @g:m@guPuP@g@p(\@g}W|W@g6i@gK~@gO贁N@gK~@gj6i@g@gj6i@g__@gףp= @gfffff@gQ@g0b/@g@gJJ@p\(\@g%I$I#@g@g @gDDDDDD"@gQ#@g:m@g۶m۶m@g @g++b$@g:m@g33333"@g@g t@ @gm۶m@g??#@p)\(#@v::+@v @vףp= %@v)\()@v_,Œ,@v@vn۶m6"@v:m'@vX|W|,@v:m:@v(@vwwwwww&@v'@v!@vr#7r#7)@ywwwwww*@v$I$ 3@vŒ_,"@vQ-@v;1@v6i4@v!@v__(@v t@.@vW|W<4@v ףp= "@v1@v(\/@v:mӆ0@vA%@v1@yn2@v%I$I8@vA t@%@v:m4@v7@v~K~:@v #@vN0@vףp= W5@vI$I;@v~K~$@vfffff5@v6i66@vQ7@vW|W(@vB/B7@y9@vuPu?@v:m'@vK~K9@vK~;@vHA@v::(%@v}W|3@vq= ףp:@v33333SA@vƒ_,%@vy;@v> ףp;@vX%=@vgffff,@v <@yY%X?@vX|W|C@vffffff*@v{Gz>@vzGA@v{GE@v+'@v::8@vK~ @@v,E@vK~(@vfffff@@v%X A@v{GzB@vL0@v$7r#7@@y贁N[C@ AE2I@֮N5;@ۻ1E@Z H@ 6KK@xӏ9@h%kA@+Id/F@HRZK@ٱ:9@KWG@)+aF@]PG@uRB>@qFWaH@(۠gI@ Dx0dJ@X=;@j&E@PG@ k          gg&p#gճCƲR@gflQ@gL@g R@g433333R@gyX R@gF8T@pc1XR@g١5K@g'B@gF'J@g! 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