Faculty Staff Graduate Studies Prospective Students Course Descriptions Extension/Outreach Research Facilities Poultry and Egg
   Publications Database

Joseph Giambrone came to Auburn University in 1977. He has directed the research of numerous undergraduate and graduate students who have gone on to careers in Laboratory Diagnostics, Pharmaceutical Laboratory Research and Academics. Dr. Giambrone developed the first CD ROM in Poultry Health and Diseases, which is available from Elsevier, Inc.

EDUCATION:

1977 - Ph.D., Microbiology, University of Georgia
1974 - M.S., Animal Science, University of Delaware
1972 - B.S., Animal Science, University of Delaware

RESEARCH:

Dr. Giambrone's current research is on viral diseases of young chickens. He is working on infectious bursal disease virus (IBVD) and reoviruses infections. These viruses cause common infections, which can result in morbidity, mortality, and/ or immunosuppression in young chickens. He has developed improved diagnostic techniques to detect the presence of these viruses in chickens using the latest molecular biological techniques such as monoclonal antibodies, recombinant probes, the polymerase chain reaction (PCR) test and sequencing of nucleic acids. Dr. Giambrone has used these reagents and procedures to develop immunoperoxidase assays, in situ hybridization assays, PCR restriction fragment length polymorphism tests, and in situ PCR hybridization tests. He has also used conventional virological techniques to develop and/or test the efficacy of vaccines against these important viral diseases of chickens.

We are developing an edible transgenic plant vaccine against IBDV in alfalfa. We choose this plant over corn and soybeans, because it is easier to transform than the latter two. We have isolated the VP2 gene from an antigenic Variant E IBDV isolate. This gene is the major immunogen of IBDV and can induce neutralizing antibody in susceptible chickens. We transformed a T-vector with the VP2 gene. This vector was made for sequencing, so we could determine that we had the proper gene. We have also transformed agrobaterium bacteria with this gene and have used this gene construct to transform aribidopis, a common weed.

TEACHING / INSTRUCTION:

POUL 4100 - Supervised Investigation
POUL 5080 - Poultry Health
POUL 5083 - Poultry Health
POUL 5084- Poultry Health
POUL 6080 - Advanced Poultry Health
POUL 7100 - Supervised Investigation
POUL 8160 - Laboratoy Techniques in Molecular Virology
POUL 8970 - Special Problems / Health
POUL 8990 - Research and Dissertation

Adv Laboratory Techniques in Avian Medicine CD-Rom
Description: Provides the Veterinarian, College Professor, and diagnostic laboratory worker, which is already experienced and equipped for diagnosis of avian diseases, more advanced and sophisticated techniques for disease diagnosis.

For purchase information, click on here.

EXTENSION / OUTREACH:

In 1980 Dr. Giambrone became an Adjunct Assistant Professor in the Department of the Microbiology in the College of Veterinary Medicine. For 6 months in 1987 he served as a visiting scientist at the CSIRO Division of Animal Health, Parkville, Australia. He was a visiting scientist in the Animal Health Research Institute, Cairo, Egypt for a month in 1993. In 1996 and 1997, he was a visiting scientist for one month in the China Agriculture University, Beijing, China.

SELECTED SCIENTIFIC PUBLICATIONS:

Guo, Z., J. J. Giambrone, Z. Liu, T. V. Dormitorio, and Wu Hongzhuyan. 2004. Effect of in ovo administered reovirus vaccine on immune responses of specific pathogen free chickens. Avian Dis. 48:224-228.

Hongzhuyan, Wu., N. K. Singh, Locy, R. D., Scissum-Gunn, K., and J, J, Giambrone. 2004. Comparison of Avian reovirus S1 genes. J. Appl. and Vet. Advances. 3 (5):325-328.

Hongzhuyan, Wu., N. K. Singh, Locy, R. D., Scissum-Gunn, K., and J. J. Giambrone. 2004. Expression of immunogenic VP2 protein of infectious bursal disease virus in Arabidopsis thaliana. Biotechnology Letters. 26:787-792.

Hongzhuyan, Wu., N. K. Singh, Locy, R. D., Scissum-Gunn, K., and J. J. Giambrone. 2004. Immunization of chickens with the VP2 protein of infectious bursal disease virus expressed in Arabidopsis thaliana. Avian Dis. 48:663-668

Wang, Chi-Young, and J. J. Giambrone, 2003. Molecular nd Phylogenetic analysis of Duck Hepatitis B virus. J. of Animal and Vet. Advances 2 (3):162-169.

Guo, Z., J. J. Giambrone, T. V. Dormitorio, and Wu Hongzhuyan, 2003. Safety and efficacy of In Ovo administration of an experimental reovirus vaccine in commercial broilers. J. of Animal and Vet. Advances 2 (7):400-403.

Guo, Z., J. J. Giambrone, T. V. Dormitorio, and Wu Hongzhuyan, 2003. Influence of a reovirus antibody complex vaccine on efficacy and of Marek’s disease vaccine administered in ovo. Avian Dis. 47:1362-1368.

Guo, Z., J. J. Giambrone, Hongzhuyan, Wu, and T. V. Dormitorio. 2003. Safety and efficacy of an experimental reovirus vaccine for in ovo administration. Avian Dis. 47:1423-1428.

Corley, M., J. J. Giambrone, and T. V. Dormitorio. 2002. Evaluation of the immune response and detection of infectious bursal disease viruses by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay after in ovo vaccination of commercial broilers. Avian Dis.46:775-802.

Corley, M., and J. J. Giambrone. 2002. Immunosuppression in specific pathogen free broilers administered infectious bursal disease virus vaccines by in ovo route. Avian Dis.46:803-809.

Wang, Chi-Young, J. J. Giambrone, and B. F. Smith. 2002. Detection of Duck Hepatits B Virus on Filter Paper by PCR and SYBR Dye-Based Quantitative PCR. J. Clinical Micro. 40:2584-2590.

Wang, Chi-Young, J. J. Giambrone, and B. F. Smith. 2002. Development of Viral Disinfectant Assays for Duck Hepatis B Virus Using Cell Culture/ PCR. J. Virol. Meth. 106:39-50.

Wang, Chi-Young, J. J. Giambrone, and B. F. Smith. 2002. Comparison of Cell Culture Systems for Duck Hepatis B Virus Using SyBr Green Quantitative PCR. J. Virol. Meth. 106:175-184.

Wang, Chi-Young, J. J. Giambrone, T. V. Dormitorio, and L. B. Allen. 2002. Nested PCR for Detecting Duck Hepatitis B Virus. J. of Animal and Vet. Advances 2:30-37.

Graduate Students

LAB LINK