Theses and Dissertations

Title: Characterization of thiamine deficiency in channel catfish (Ictalurus punctatus, Rafinesque) fed heated and non-heated catfish processing waste

Name: Camacho, Arsenio Sabangan

Degree: PhD

Chair: Dr. Richard T. Lovell

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1974

Pages: 85

Keywords: Thiamine Deficiency,Channel Catfish,Feeds,Catfish Processing Waste.


Channel catfish fingerlings, stocked at 6,000 per acre in 0.005-acre concrete tanks were fed 32% protein, nutritionally adequate, moist fermentation-preserved feeds containing raw or heated catfish waste for 120 days. Fish were also fed diets prepared in dry, pelleted form containing catfish waste or fish meal. Fish fed the fish meal diet gained approximately 20% more than those fed any of the rations containing catfish waste. Gain by fish fed preheated waste in a moist, fermentation-preserved diet was equal to that by fish fed the waste in a dry, pelleted feed, indicating that moist, fermentation-preserved feeds are suitable for feeding catfish. Slightly poorer growth response by fish fed the raw waste diet indicated that catfish waste possibly contains a heat labile anti-nutritional factor. A subsequent feeding trial was conducted in which channel catfish fingerlings were fed diets containing raw or heated waste to which no thiamine was added exhibited a syndrome associated with thiamine deficiency. Chemical tests showed that catfish skin and viscera contained a heat labile anti-thiamine factor but its activity was low. Low pH of 4.5 associated with fermentation did not inactivate the anti-thiamine factor. These studies indicate that waste from catfish is severely deficient in thiamine. If the waste is not heated prior to mixing in a fish feed, the thiamine supplement should be fed in a separate feed.

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