Theses and Dissertations

Title: Continuous-flow electrophoretic fractionation of phytoplankton suspensions

Name: Bayne, David Roberge

Degree: PhD

Chair: Dr. John M. Lawrence

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1970

Pages: 93

Keywords: Continuous-Flow Electrophoretic Fractionation,Phytoplankton Suspensions.


The Beckman Continuous Particle Electrophoresis (CPE) System was evaluated as a means of physically isolating components of mixed phytoplankton suspensions. Twenty-one freshwater phytoplankton genera ranging in greatest average dimension from 1 to 405 u were electrophoretically treated. All algal cells examined migrated toward the anode in the electric field and different mobilities frequently resulted in fractionation of mixtures of different genera. Organic debris, clay particles and bacteria often exhibited distinct mobilities that led to their separation from other constituents. Akinetes were separated from motile vegetative cells of the genus Haematococcus. A 0.001M tris buffer, tris (hydroxymethyl) aminomethane, pH 9.2, resulted in greater particle mobility and better resolution than a 0.001M barbital buffer, sodium diethylbarbiturate, pH 8.6. Increase in buffer pH tended to make algal particles more negative and increased mobility. A direct relationship existed between applied field gradient and particle mobility. Resolution generally improved at higher field gradients. The effectiveness of the separation procedure was limited on occasions by certain algal properties such as motility, size, formation of aggregates and bouyancy.

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