Theses and Dissertations

Title: Response of Nile Tilapia (Oreochromis niloticus) Fed Diets Containing Selected Mycotoxins

Name: Tuan, Nguyen Anh

Degree: PhD

Chair: Richard T. Lovell


University: Auburn University

Location: Auburn, Alabama

Date: 2001

Pages: 110

Keywords: nile tilapia,mycotoxins,AFB1,FB1,MON,diet,feed


Responses of Nile tilapia (Oreochromis niloticus) to subchronic and toxic concentrations of moniliformin (MON), fumonisin B1 (FB1) and aflatoxin B1 (AFB1) were evaluated under controlled environmental conditions. Nile tilapia (2.7 g) were fed semi-purified diets containing MON at concentrations of 10, 40, 70, 150 mg/kg: FB1 at 10, 40,70, 150 mg/kg: and AFB1 at 0.25, 2.5, 10, 100 mg/kg, for 8 weeks. The mycotoxins were obtained from Fusarium proliferatum (for MON), F. moniliforme for (FB1), and Aspergillus flavus (for AFB1) culture materials. Fish fed diets containing MON at concentrations of 70 mg/kg or higher, FB1 at concentrations of 40 mg/kg or higher and AFB1 at concentrations of 2.5 mg/kg or higher had lower weight gains than the control fish. There was no difference in survival rate among treatments except for fish fed diets containing 100 mg of AFB1/kg which had lower survival. Serum pyruvate level was increased in all fish fed MON. The diet containing the 150 mg/kg of FB1 caused a higher ratio of free sphinganine to free sphingosine in livers compared to the control and other diets. Histological examination revealed no tissue lesions in fish fed diets containing MON or FB1. However, fish fed AFB1 at 10 mg/kg of diet had different sizes of hepatocyte nuclei, lipofuscin and necrotic lesions. Pleomorphic nuclei, lipofuscin, and necrosis were commonly observed in liver of fish fed 100 mg/kg of AFB1. Preneoplastic lesions with basophilic spindle-shaped cells, larger and pleomorphic nuclei, and destruction of hepatic tissue were observed in liver of fish fed 100 mg/kg of AFB1. This research indicates that, compared to other warmwater fish species, Nile tilapia are equally and moderately sensitive to MON and FB1 but appear to be highly sensitive to AFB1.

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