Theses and Dissertations


Title: The digestive enzymes of the bluegill bream, Lepomis macrochirus, Rafinesque

Name: Lawrence, Faye Buttram

Degree: MS

Chair: Dr. Homer S. Swingle

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1950

Pages: 46

Keywords: Digestive Enzymes,Bluegill Bream,Lepomis macrochirus Rafinesque.

Abstract:

An investigation of the enzymes produced by the digestive tract and its associated glands in the bluegill bream, Lepomis macrochirus Rafinesque, was made to provide more information concerning the physiology of digestion in fishes. Analyses of tissue extracts and digestive-tract contents were made according to standard procedures to determine the sites of secretion and of digestion by the principal enzymes important in alimentary processes. There was a complete absence of digestive activity by the esophagus. Of the four carbohydrases studied, amylase was identified in extracts of the liver. Invertase was found in tissue extracts of the pyloric caeca and extracts of the intestinal contents. The pyloric caeca and the liver secreted maltase which was active in the duodenum and intestine. All tests for the presence of lactase were negative. The fat-splitting enzyme, lipase, was present in the liver, pancreas and pyloric caeca and in the lumina of the duodenum and intestine. Pepsin was found in the tissue extracts and in the contents of the stomach. Trypsin occurred in the tissue extracts of the pancreas, pyloric caeca, and duodenum and in the contents of the duodenum and intestine.

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