Theses and Dissertations

Title: Tricaine methanesulfonate (MS 222) as an anesthetic for some common pond fishes

Name: Webb, Jr Robert Taylor

Degree: MS

Chair: Dr. H.S. Swingle

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1954

Pages: 55

Keywords: Trichodina,Freshwater,Southeastern United States.


The effectiveness of M. S. 222 (tricaine methanesulfonate) as an anesthetic was tested on the golden shiner, Notemigonus crysoleucas (Mitchill): goldfish, Carassius auratus (Linneaus): bluegill, Leponis macrochirus (Rafinesque): and fathead, Pimephales promelas Rafinesque. Concentrations of 0.1, 0.2, 0.4 , and 0.8 gm./gal were used. The effect of M. S. 222 was essentially the same at temperatures of 50, 60, 70, and 80 degrees Fahrenheit. The minimum concentration at which golden shiner, goldfish, bluegills, and fatheads were anesthetized was 0.4 gm./gal. with an exposure time of 2 minutes for the golden shiners, 8 minutes for the goldfish and bluegills, and 4 minutes for the fatheads.The fish were kept in aquaria for three days after being anesthetized in order to observe the aftereffects of M. S. 222. It was found that fatheads suffered from 80 to 100 per cent mortality when exposed to concentrations of 0.4 gm./gal, or higher. The other speices tested seemed to be unaffected. The minimum lethal concentration at a temperature of 84 degrees Fahrenheit and an exposure time of 15 minutes were as follow: Golden shiner..0.6 gm./gal. Goldfish..2.4 gm./gal.Bluegills.0.8 gm./gal.Fatheads.0.4 gm./gal.,MS 222,Anesthetic.

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