Theses and Dissertations


Title: Effect of various chemicals on survival of crowded fathead minnows in jars and plastic bags

Name: Pongsuwana, Umpol

Degree: MS

Chair: Dr. E.E. Prather

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1960

Pages: 99

Keywords: Chemicals,Survival,Fathead Minnows,Crowded,Jars,Plastic Bags.

Abstract:

Laboratory experiments were conducted with 7 chemicals to determine their effect on the survival of fathead minnows, Pimephales promelas Raf. under simulated conditions of transportation. Experiments were conducted in open glass jars at room temperatures of 72o to 90o F. The chemical that proved to be most beneficial was then tested in closed plastic bags using oxygen at atmospheric pressure. Observations on survival were made at regular intervals, but dead fish were not removed until the experiment ended. Sodium amytal was found to be toxic above 50 p.p.m., quinaldine above 5 p.p.m., methyl pentynol above 600 p.p.m., and tricaine methane sulfonate above 40 p.p.m. Each anesthetic was tested at concentrations lower than the toxic levels. Sodium amytal at 12 and 16 p.p.m., quinaldine at 2 and 4 p.p.m., methyl pentynol at 500 and 600 p.p.m., and tricaine methane sulfonate at 40 p.p.m., each failed to increase survival rate of the fathead. The buffer, Sigma 7-9, at concentrations of 250, 500, 1000, and 1500 p.p.m. did not increase survival rate of the fathead. Acriflavine at 1 p.p.m. and activated charcoal at 198.4 grams per 1750 ml. of water showed beneficial effects in increasing survival rate of the fathead in open jars. Combinations of charcoal plus acriflavine and charcoal plus sodium amytal were not significantly better than charcoal alone, but were better than acriflavine alone and sodium amytal alone. Activated charcoal was also effective in increasing survival rate of the fathead in closed plastic bags with oxygen at atmospheric pressure.

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