Theses and Dissertations

Title: Production of The Copepod Apocyclops panamensis Under Hatchery Conditions

Name: Lipman, Emily Elizabeth

Degree: MS

Chair: Ronald P. Phelps


University: Auburn University

Location: Auburn, Alabama

Date: 2001

Pages: 68

Keywords: larval feed,copepod,hatchery production


Copepod nauplii are an important early food for marine fish larvae and a key ingredient in the hatchery production of several fish species. However, the use of copepod nauplii as a live feed is limited because of the difficulties in their controlled production. This research was divided into four studies. The first two studies helped establish procedures for the intensive production of copepods in a hatchery. The third study looked at optimizing production of Apocyclops panamensis by altering stocking density of adults. The fourth and final study investigated the feasibility of a closed batch production system.The first study investigated the appropriate culture species, Acartia spp. versus A. panamensis. Copepods were cultured in 100 L bags filled with 40 L of 30 ppt artificial saltwater and seeded with Isochrysis galbana at 5 X 105 cells/mL. Five bags for each treatment were stocked with Acartia spp. or A. panamensis adults each at a density of 320/L. After 14 days, the cultures were harvested and A. panamensis, with a mean production of 773.4 ? 140.4 nauplii and copepodites/L, was determined to be a better culture species than Acartia spp., 324.6 ? 57.4 nauplii and copepodites/L.The second study considered the appropriate density to seed I. galbana in the culture bags. The culture system was the same as described above, but only A. panamensis adults were stocked at 320/L. Two algae densities were tested, 5 X 105 cells/mL and at 1 X 106 cells/mL. The bags were harvested after they cleared, 8-9 days after stocking. There were no significant differences in production of nauplii and copepodites/L between the two treatments.In the third study, adult copepods (A. panamensis) were stocked at 320, 640, 1,280, 2,560, and 5,120L into 100 L culture bags. These bags contained 40 L of 30 ppt artificial seawater and I. galbana at 5 X 105 cells/mL. The first trial tested 320 adults/L, the second trail 640 and 1,280 adults/L, the third 1,280 and 2,560 adults/L, and the final trial tested 2,560 and 5,120 adults/L. The results show that A. panamensis can be raised at high densities with production up to 17,872.5 ? 654.0 nauplii and copepodites/L and 8.6 ? 0.9 nauplii and copepodites/female.In the fourth study, copepods were initially trapped from the wild and stocked into culture bags prepared as described above. All subsequent cultures were stocked with adults harvested from the previous culture. Peak production from this system (36,800 nauplii and copepodites/L) was substantially higher than the largest production numbers (19,095 nauplii and copepodites/L) attained from previous studies using wild adults. Females in this study produced on average 13.3 ? 2.5 nauplii and copepodites.

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