Theses and Dissertations

Title: Genetic resource analysis of cultured Channel Catfish (Ictalurus Punctatus) in Alabama using amplified fragment length polymorphism (AFLP) markers

Name: Mickett, Kathryn Dawn

Degree: MS

Chair: Zhanjiang Liu


University: Auburn University

Location: Auburn, Alabama

Date: 2002

Pages: 129

Keywords: Genetic resource analysis,Channel Catfish (Ictalurus Punctatus),amplified fragment length polymorphism (AFLP) markers


Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of domestic channel catfish (Ictalurus punctatus). Sixteen populations within Alabama were surveyed using 5 primer combinations. A total of 454 reproducible, polymorphic bands were detected from 282 individuals, with an expected mean heterozygosity of 0.135. The percentage of polymorphic loci varied greatly within distinct populations, from 18.3% to 100%. The populations of research stocks, including the albino stock and the random research stock, had lower percentage polymorphic loci than the farm-raised populations. This difference was possibly due to selection and reduced population size of the research stocks. The estimated average Fst value across all loci was 0.4456. However, this value dropped to 0.1763 when population 16 was excluded from the analysis. Cluster analysis by Jaccard's pairwise similarity coefficient indicated high similarity among individuals, with few strong population-specific clusters except for population 16. Nei's population-wise identity measures found all populations but population 16 highly related with 96% similarity. Population 16 was 34% similar to the other populations. The results of this study suggested that most, but not all, brood stocks of channel catfish used in the aquaculture industry in Alabama have similar genetic background. Such information has implications for future brood stock selection and management and for further analysis of interactions between domestic and wild populations of channel catfish.

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