Theses and Dissertations

Title: A survey of methods in artificial breeding of grass carp

Name: Ventura, Rodolfo Feijo

Degree: MS

Chair: Dr. William L. Shelton

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1974

Pages: 41

Keywords: Grass Carp,Artificial Breeding.


Experiments on artificial breeding of grass carp were conducted in 1973 and 1974 at two locations with different water sources: pond water accompanied with silts was the source for the 1973 experiment: and clear dechlorinated tap water for the 1974 experiment.Observations on the external features of the spawners and the characteristics of their sampled ova by means of a catheter were made. Along with this, studies were done on the effect of three types of hormones. The general condition of the broodstock of 1973 and 1974 from handling prior to initiation of spawning were compared. And finally, fertilization and hatching problems were noted. Results indicate a relationship between the external features and characteristics of the sampled ova. Pituitary and a combination of HCG and pituitary hormonal injection were relatively effective in inducing ovulation among ready recipients whose ova were loosely attached. Eggs from these females swelled in water in a period of five minutes. The same recipients were given relatively pro-per treatment four months prior to the 1974 experiment. With two exceptions, the 1973 group of spawners that were kept overcrowded in ponds did not respond to the hormones. Some of the spawners exhibited characteristics of a ready recipient. Ovulation time can be predicted by observing the behavior of the fish. Trembling behavior in the female as she approaches ovulation is a positive sign. Flowing milt can be made available eighteen hours after male is injected with a heavy dosage of pituitary. Percentage of fertilization was high among eggs fertilized by flowing milt but mass mortality was experienced six hours after fertilization. A low percentage of hatching was accomplished among eggs that were freshly released and immediately fertilized but none on eggs from female that already partially ovulated in tank.

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