Theses and Dissertations

Title: Demonstration of an immune response in the channel catfish, Ictalurus punctatus (Rafinesque), to Chondrococcus columnaris (Davis)

Name: Schachte, Jr John H.

Degree: MS

Chair: Dr. E.W. Shell

Resides: FAA Library

University: Auburn

Location: Auburn, Alabama

Date: 1972

Pages: 40

Keywords: Immune Response,Channel Catfish,Chondrococcus columnaris.


In December 1970, an experiment was initiated to attempt to induce an immune response in the channel catfish Ictalurus punctatus against Chondrococcus columnaris. The investigations were carried out in the laboratory of the Southeastern Cooperative Fish Parasite and Disease Project, Department of Fisheries and Allied Aquacultures, Auburn University. An injectable antigen was prepared and administered to the fish using two routes of injection. One group of catfish received intramuscular injections, while the second group was injected subcutaneously. This procedure was followed in an attempt to determine if any difference in titer could be expected based on the route of injection. After a 3 week period had elapsed following initial injections, blood samples were drawn and titers quantified according to the slide agglutination technique. An antigen preparation stained with toluidine blue was used to achieve sharper contrast. All but one control fish in the experiment displayed no naturally occurring titer against C. columnaris. This fish had a titer of 1:160. The remaining test fish of both the intramuscular and subcutaneously injected groups had an average titer of 1:4337. Statistical analysis indicated that no significant difference in titer between the two routes of injection was present. This data and corresponding work in cold water fishes are compared and discussed.

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