The Fish Molecular Genetics & Biotechnology Laboratory

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Purpose:
Utilizing genetics and genomics for the study and improvement of aquatic organisms. 

Contact Information:
Dr. Zhanjiang (John) Liu
(334)-844-4054 (office)
(334)-844-4085 (lab)
(334)-844-9208 (fax)
zliu@acesag.auburn.edu


Staff, Students, & Scholars
The Fish Molecular Genetics & Biotechnology Lab Director
Zhanjiang (John) Liu, Ph.D.
Research Interest
Genomics, Transgenics, Marker-assisted Selection and DNA Marker Technologies.

Visiting Scholars

Graduate Students Completed :

Name Degree(Year)/Major Current Position
Putharat Baoprasertkul Ph.D. (2006)/Fisheries Fisheries Biologist 5, Aquatic animal Genetics Research and Development, Department of Fisheries, Ministry of Agriculture, Pathumthani, Thailand
Eric Peatman M.S. (2004)/Fisheries Ph.D. student at Auburn University
Micah Simmons M.S. (2003)/Fisheries Research Assistant at USDA ARS Aquatic Animal Health
Jerry Serapion M.S. (2003)/ Fisheries The Phillipines
Attila Karsi Ph.D. (2001)/ Fisheries and CMB Postdoc, College of Veterinary Medicine, Mississippi State University
Arif Kocabas Ph.D (2003)/Fisheries and CMB Postdoc, Cellular Reprogramming Laboratory Department of Animal Science, Michigan State University
Zhenlin Ju Ph.D (2001)/Fisheries and CMB
Kathryn Mickett M.S. (2001)/Fisheries and CMB Research Associate II, Fishery Biology, AuburnUniversity
Andrea Patterson M.S. (2001)/Fisheries and CMB Research associate, Texas Medical School at Houston
Soonhag Kim Ph.D. (2000)/Fisheries and CMB Postdoctoral fellow at West Virginia University
Guo Tan M.S. (1999)/Fisheries and CMB Research Associate at Harvard University
Attila Karsi M.S. (1998)/Fisheries and CMB Ph.D. student at Auburn University
Anang Kristanto Ph.D. (2004) Fisheries Government Fisheries Department, Indonesia
Sara Thacker M.S. (2004)/Animal Science
Stuart Goong Ph.D. (2004)/Fisheries
Zhuyan Guo Ph.D. (2003)/Poultry Science Postdoc at Tufts University
George Osure M.S. (2003)/Fisheries Researcher in Kenya
Joseph Padi Ph.D. (2003)/Fisheries Postdoc, Southern Fresh
Julia Pridgeon Ph.D. (2002)/Entomology Postdoctoral Fellow, Auburn University
Justin Evans M.S. (2002)/Biological Sciences Research Associate, Auburn University
Tulin Arslan Ph.D. (2001)/Fisheries Assistant Professor, Mugla University, Turkey
Phillip Waters M.S. (2001)/Fisheries Ph.D. student, Auburn University
Dave Wohlford M.S. (2001)/Animal Science Scientist with a breeding company
Wencai Yang Ph.D. (2000)/Agronomy Postdoctoral fellow at University of Rochester
Dayton Lambert M.S. (1998)/Fisheries Peace Corp in Africa
Amy Nichols M.S. (1998)/Fisheries and CMB Research associate at Clemson University
Soonhag Kim M.S. (1996)/Fisheries Postdoctoral fellow at West Virginia University
Chitmanat Chanagun M.S. (1996)/Fisheries Ph.D. student at University of Georgia
Ping Lang Ph.D. (2004)/Horticulture Postdoc at Auburn University
Zhinong Yan Ph.D. (2000)/Plant Pathology Postdoctoral Fellow at University of Georgia
Brad Argue Ph.D. (1996)/Fisheries Scientist at Oceanic Institute, Hawaii

Current Graduate Students:

Name Department Degree
Benjaporn Somridhivej Fisheries M.S.
Putharat Baoprasertkul Fisheries Ph.D.
Shaolin Wang Fisheries Ph.D.
Jason Abernathy Fisheries Ph.D.
Peng Xu Fisheries Ph.D.
Eric Peatman Fisheries Ph.D.
Qiang Xu Entomology and Plant Pathology Ph.D.
Fang Zhu Entomology and Plant Pathology Ph.D.
Atra Chaimongkol Fisheries Ph.D.
Jiarong (Rona) Liu Horticulture M.S.
Cankui Zhang Horticulture Ph.D.
Michael Trask Fisheries M.S.
John Arndt Fisheries M.S.
Catherine Nordfelt Fisheries Ph.D.
Frost Rollins Horticulture Science M.S.

Undergraduate Students:

Name Major University
Jackson Hayes Fisheries Auburn University
Jarrod Cody Young Fisheries Auburn University
Sam Pack Fisheries Auburn University
Timothy Carpenter Fisheries Auburn University
Jennifer Fisheries Auburn University
Timothy McLean Fisheries Auburn University
Abel Carrias Fisheries Auburn University
Tommy Purcell Fisheries Auburn University
Micah Simmons Fisheries (trainee) Auburn University
Trevor Silvernail Molecular Biology Auburn University
Kevin Roberg Molecular Biology University of Minnesota
Bob Iwine Cell Biology University of Minnesota


Facilities & Equipment


The 6500 square foot Fish Molecular Genetics and Biotechnology Laboratory contains all the equipment necessary for analysis and manipulations of nucleic acids and proteins. The laboratory includes a main laboratory (radioactive safety approved), refrigerated room, walk-in freezer, dark room, tissue culture room, an attached conference room, and two attached offices.

The major equipment in thelaboratory include: two automated DNA sequencers equipped with sequencing, genotyping, and fingerprinting apparatus and software, one pyrosequencing equipment with pyrosequencing, genotyping software, a light-cycler system for quantitative gene expression analysis, one CCD Gel Imaging System equipped with various gel analysis modules and software, one scintillation counter, one autoclave, four PCR thermocyclers, one MicroPulser electroporator, One UV linker Microwave 2400, one microinjection system with micromanipulator controlled by a pico-injector Model PLI-100, a Bakeonizer for electroporation, a UV-visible spectrophotometer, one ice maker, a clinical centrifuge, a centrifuge, a GS-15 centrifuge, five microfuges, two computerized power supplies, 15 regular power supplies, two 37°C CO2/humidified incubators, two -80°C freezers, one -20°C freezer, one analytical balance, one 37°C shaker incubator, one 37°C incubator for dish culture, UV and visible transilluminators with camera, a Leitz microscope, two dissecting microscopes, and three biosafety cabinets, one of which is devoted as a radioactive work station. The laboratory has all the equipment for molecular biology: molecular cloning, PCR, restriction analysis, gel electrophoresis, DNA sequencing, blotting, hybridization, gene expression studies of RNA and proteins, radioimmunoassays (RIA), ELISA, and Western blotting.

We have a several computers and printers for routing computing operations; both PC and Mac platforms are available. We have several DNA analysis software packages and fragment analysis software such as GelExpert, AFLP-Quantar- Pro, Gene ImageIR genotyping softwares, access to GenBank, EMBO, and other databases, and to GCG software packages. Auburn University also has excellent Genomics and Sequencing facilities with one ABI Prism 3100 capillary sequencer and various software packages, Taqman and older models of ABI automated DNA sequencers, which are available at a minimal cost.

Auburn University has the best facility in the nation for simulated commercial conditions for catfish genetic research. A large earthen pond facility consists of 78 experimental research ponds is available for evaluation of performance under closely simulated commercial conditions for QTL analysis. Fifty additional ponds are available for control fish, and other related experiments. This is important since genotype-environment interactions can occur in genetic evaluations of catfish necessitating pond experiments to obtain realistic results. An on-site hatchery and modern genetics laboratory complement the earthen ponds.

The 6500 square foot hatchery contains 300 tanks for indoor spawning, incubation and disease challenges and resistance evaluations. The hatchery is equipped with heating and cooling systems to manipulate temperature of the hatchery.


Research

DNA marker technologies: The Fish Molecular Genetics and Biotechnology Laboratory at Auburn University is one of the most active labs in development and application of DNA fingerprinting technologies. We have developed markers including isozyme markers, restriction fragment length polymorphic (RFLP) markers, random amplified polymorphic DNA (RAPD) markers, amplified fragment length polymorphic (AFLP) markers, polymorphic expressed sequence tag (EST) markers, microsatellites or simple sequence repeat (SSR) markers, and single nucleotide polymorphic (SNP) markers, for applications in various fish species, especially in catfish. For catfish, we have developed over 600 RAPD markers, over 3,000 AFLP markers, over 2500 microsatellite markers, and several hundreds of SNP markers. We have thoroughly evaluated the usefulness, inheritance, and adaptability of these markers in catfish.

Development of type I markers: Recently, our laboratory has developed two ways for efficient development of type I markers. The first is the identification of microsatellite containing cDNA clones. In our brain EST work, we have identified 122 EST clones (about 10% of sequenced clones) with microsatellites. Similarly, many ESTs from the head kidney, spleen, and skin cDNA libraries also contained microsatellites although at a lower percentage. In the second approach, we have adopted a comparative EST analysis in channel catfish and blue catfish. Essentially in all ESTs analyzed, there is at least one SNP in each EST. We have identified over 100 type I SNPs. Mapping of these type I markers should set the foundation for integration of linkage maps and large scale comparative genomics.

Resource families: Although channel catfish is the major cultured catfish, the channel catfish x blue catfish hybrid system offers great advantages. The F1 hybrid is fertile and in fact we have produced F2, F3, and various backcrosses (Argue 1996, Liu et al., 1997; Dunham et al., 1998). They are a major resource for QTL mapping because of high levels of marker polymorphism and drastic difference in phenotypes. In terms of disease resistance, channel catfish is superior in resistance to columnaris disease (caused by Flavobacterium columnare), while blue catfish is superior in resistance to enteric septicemia of catfish (ESC, caused by Edwardsiella ictaluri) (Dunham et al., 1993a). ESC and columnaris are the two most severe diseases in catfish accounting for over 78% of the disease problems (NAHMS, 1997). In terms of processing yield, blue catfish is superior to channel catfish, providing 5-8% more fillet yield than channel catfish. This interspecific system, therefore, provides a model system for analysis of major QTLs involved in disease resistance and processing yield.

Genomic Organization and Composition: Channel and blue catfish both have 29 pairs of chromosomes with 1.0 X 109 bp per haploid genome (LeGrande et al., 1984; Tiersch and Goudie 1993). Using AFLP analysis, we have determined that the catfish genome is highly A/T-rich (Liu et al., 1998c). In searching for satellite and minisatellite sequences, a class of A/T-rich elements known as Xba elements was characterized. The Xba elements are highly abundant accounting for 5-6% of the catfish genome (Liu et al., 1998); We also characterized several classes of Tc1-like transposable elements, of which a non-autonomous element has high copy numbers accounting for 1.6% of the catfish genome (Liu et al., 1999). Recently, we have found two classes of short intersperse elements (SINE) termed as the Mermaid and Merman elements. These two types of SINEs are specifically associated with active genes of aquatic animals and may have played major roles in shaping the aquatic genomes (Kim et al., 2000).

Genetic linkage mapping: We have mapped 563 AFLP markers and constructed the first generation genetic linkage map in catfish that include 43 linkage groups with a genomic coverage of 2,450 cM. We have genotyped over 300 microsatellite markers and linkage analysis is under way. Our work in linkage mapping represent the first genetic linkage map constructed using channel catfish x blue catfish hybrid system.

Mapping of quantitative trait loci (QTL): One of our research objectives is to determine the genes involved in various performance and production traits and map their chromosomal locations. We are very interested in genes controlling growth rate, feed conversion efficiency, disease resistance, and processing yields. To date, we have identified one marker linked to growth rate, three markers that are linked to feed conversion efficiency, and several markers that are linked to disease resistance to enteric septicemia of catfish. Genome-wide QTL scan using AFLP markers has been conducted and the data analysis is under way. Fine mapping of disease resistance QTLs are in progress.

Transcriptome analysis of catfish: Aiming at understanding the major proportion of the entire catfish transcripts (transcriptome), we have initiated large-scale analysis of expressed sequence tags (ESTs). To date, we have established 24 cDNA libraries and 4 normalized cDNA libraries. We have sequenced over 43,000 ESTs from catfish representing over 25,000 unique genes. Our goal is to sequence ESTs from all of the catfish tissues using both normalized and subtracted libraries to establish a Unigene set for functional genomics research. We have already used the existing ESTs and conducted microarray analysis to identify differentially expressed genes under biotic or abiotic stresses. In addition to catfish ESTs, we have produced 4,348 ESTs from eastern oysters (Crassostrea viginica). A total of 41,867 entries have been deposited to GenBank from our laboratory.

Expression vectors: We have isolated many fish promoters and constructed the first “all-fish” expression vectors. Our goal is to develop technology that provides approaches for safer and more efficient gene transfer. In a recent collaboration with Dr. Boaz Moav in Israel, we have constructed new generations of expression vectors with both the ability for early integration, and stable transgene expression by combining the border elements and the Sleeping Beauty transposon technologies together.

Cloning of economically important genes: We have cloned various important genes from catfish that may be useful for biotechnology including growth hormone, gonadotropin alpha subunit, gonadotropin beta subunit 1, gonadotropin beta subunit 2, proopiomelanocortin, myostatin, and many other genes that are potentially important for application in aquaculture biotechnology.
Genetic resource analysis: We are conducting research to understand the genetic variation of domestic catfish. We are also conducting research on genetic resources of catfish in the wild populations in Alabama, using AFLP technology.

Systematic Analysis of Ribosomal Proteins of Channel Catfish: We have cloned, sequenced and characterized all 79 ribosomal protein mRNAs and their expression including 47 60S ribosomal protein genes and 32 40S ribosomal protein genes. Together, with exception of the human, mouse, and rat genes, the 79 channel catfish ribosomal protein mRNAs represent the most complete set of ribosomal protein gene sequences from a single organism that should be useful for phylogenetic and comparative genomic studies.

Fish Population Genetics: There is little information on the genetic characteristics of fish in natural waters, and the inheritance of those characters. More data need to be collected and disseminated to all parties involved in fisheries science. Procedures for preservation of natural gene pools need to be developed. Strain evaluation, hybridization, polyploidy and sex-reversal in fish need to be evaluated. Also problems of genetic inbreeding should be addressed. Assessment of genetic resources will enable us to develop programs to preserve and evaluate the genetic integrity. Additionally, these problems will lead to improvement in fishing and alleviate problems associated with fishing pressure and altered habitat. Using AFLP markers, we have assessed the genetic resources of both domestic and wild catfish populations. We have also determined the genetic impact of domestic catfish on their wild populations.
The main objective of the Southeastern Cooperative Regional Project on Fish Genetics and Breeding of Fish is genetically preserve natural gene pools and/or improve cultured sport fish in cooperating states, understanding genetics of certain species such as endangered species. The long-term objectives are to preserve the genetic diversity while enhancing natural resources for fisheries as well as aquaculture. Click here to go to Southeastern Cooperative Fish Genetics pages.

Environment Biotechnology/Environmental Genomics: We just initiated a project aimed at analysis of differentially expressed genes upon exposure of organism to adverse environment. We are particularly interested in impact of environmental pollution on gene expression. Toward this end, we choose oyster as the model organism. Oysters are found throughout coastal marine ecosystems in the United States. Our study will utilize the Eastern oyster, Crassostrea virginica. Oysters are sedentary and thus are good representations of a particular location. They are filter feeders, feeding directly on particulate organic matter and phytoplankton. They reach sizes in less than a year which are practical for analytical purposes. Oysters have a longevity of 5-7 years so that they are also effective bioindicators for a relatively long time. Since we are interested in potential impact of pollutants on reproduction, particularly sex phase switch and sex ratios, we will be using oyster gonad tissues and gill tissues to provide accurate molecular information concerning genomic expression signatures.

Application of microarray technology in aquatic systems: Our laboratory is applying microarray cDNA technology to address fundamental question concerning gene regulation with regard to development, physiology, and stress biology. We have developed microarray technology and used the technology to address physiological questions for both the normal biology and stressed biology. Our key interest is to understand genes and their expression during and after infection by pathogens. As our transcriptome analysis progresses, we will be able to produce microarray technologies for tissues, organs, and biochemical pathways. In a recent study, we have determined genes that are differentially expressed for temperature acclimation, particularly under the cold (11C).

The catfish hybrid initiative: The Department of Fisheries and Allied Aquacultures at Auburn University is lunching a research initiative attempting to resolve the problem of mass production of the channel catfish female x blue catfish male hybrid. This specific hybrid show strong heterosis and superior performance traits in disease resistance, growth, feed conversion efficiency, growth rate, processing yields, and seinability. However, due to reproduction isolation, it has been difficult to produce mass quantities to meet the demands of the catfish industry. This initiative take an institutional approach by organizing reproductive physiologists, molecular biologists, breeders, nutritionists, aquaculturists, and all other possible elements into the team to achieve the objective of resolving the problem in five to 10 years.

Genomic Composition: To understand the genomic landscape of catfish, we have identified a family of repetitive elements known as the Xba elements. The Xba elements are arranged in head-to-tail tandem arrays, is highly A/T-rich (67%), 327-331 bp in size, and is highly similar, but not identical to one another in sequence. It has high copy numbers and accounts for about 5% of the catfish genome. We have characterized multi-family of Tc1 transposable elements. One family, named Tip1, is 1.6-kb long, low copy numbers, and is similar to Tc1 elements from other teleost fish. The second family named Tip2 is 1.0-kb in size and is more similar to Tc1 elements from invertebrates. The third family named Tipnon, is 0.5-kb in size and does not harbor transposase-related coding capacity and therefore, is non-autonomous family of transposons. Tipnon has high copy numbers and accounts for approximately 1.6% of the catfish genome. We have also characterized several families of SINE elements, particularly the Mermaid and Merman elements that are specifically associated with aquatic genomes.

Physical mapping of the catfish genome: We have initiated a physical mapping project aiming at two objectives: 1. to construct the bacterial artificial chromosome (BAC) contigs using restriction fingerprinting; and 2. to assign genes into BAC using two-dimensional hybridization. The physical mapping will set the foundation for comparative mapping the catfish genome, and for eventual complete sequencing the catfish genome.

Transgenic fish research: I started my doctorate education working on transgenic northern pikes and walleye in 1985. The Minnesota Transgenic Fish Group was one of the first groups who produced transgenic fish. We have isolated many fish promoters and constructed the first “all-fish” expression vector in 1989. We have successfully produced transgenic northern pike (1988), zebrafish (1990), and catfish harboring growth hormone genes and various reporter genes. In a recent collaboration with Dr. Boaz Moav in Israel, we have constructed new generations of expression vectors with both the ability for early integration, and stable transgene expression by combining the border elements and the Sleeping Beauty transposon technologies together.

Characterization of the selenoproteome of catfish: Twenty five genes have been reported to be included in the selenoproteome of humans. Using a systematic approach, we have identified and characterized 23 selenoprotein genes from channel catfish, making channel catfish the second organism from which almost all selenoprotein genes are cloned and characterized.

Characterization of fish innate immunity system: Although many genes involved in the innate immune systems have been cloned from mammals, it is still not completely understood if fish have similar systems. In an attempt to clone, sequence, and characterize genes involved in the fish innate immunity system, we have recently characterized 26 CC chemokines and 7 CXC chemokines from catfish. Our results suggest rapid gene duplications of fish chemokines. We have also characterized a number of antimicrobial peptide genes including NK-lysin, bactericidal permeability-increasing protein (BPI), hepcidin, liver-expressed antimicrobial peptide 2 (LEAP-2). These genes may have potential for applications in genetic improvements of catfish.


Title Journal Year
Alternative complement pathway of channel catfish (Ictalurus punctatus): Molecular characterization, mapping and expression analysis of factors Bf/C2 and Df Fish & Shellfish Immunology 2012
Molecular characterization of three L-type lectin genes from channel catfish, Ictalurus punctatus and their responses to Edwardsiella ictaluri challenge Fish & Shellfish Immunology 2012
Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus) Research in Veterinary Science 2012
Pathogen recognition receptors in channel catfish: I. Identification, phylogeny and expression of NOD-like receptors Developmental and Comparative Immunology 2012
Rapid development of molecular resources for a freshwater mussel, Villosa lienosa (Bivalvia:Unionidae), using an RNA-seq-based approach Freshwater Science 2012
Susceptibility of channel catfish, blue catfish and channel×blue catfish hybrid to Ichthyophthirius multifiliis Aquaculture 2011
Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge Fish & Shellfish Immunology 2011
Molecular responses of ceruloplasmin to Edwardsiella ictaluri infection and iron overload in channel catfish (Ictalurus punctatus) Fish & Shellfish Immunology 2011
Molecular characterization and expression analysis of the channel catfish cathepsin D genes Fish & Shellfish Immunology 2011
Microfibrillar-associated protein 4 (MFAP4) genes in catfish play a novel role in innate immune responses Developmental and Comparative Immunology 2011
Proliferating cells in suborbital tissue drive eye migration in flatfish Developmental Biology 2011
Gene expression profiling of a fish parasite Ichthyophthirius multifiliis: Insights into development and senescence-associated avirulence Comparative Biochemistry and Physiology, Part D 2011
Development of genomic resources in support of sequencing, assembly, and annotation of the catfish genome Comparative Biochemistry and Physiology, Part D 2011
Molecular responses of calreticulin genes to iron overload and bacterial challenge in channel catfish (Ictalurus punctatus) Developmental and Comparative Immunology 2011
Structure and expression of transferrin gene of channel catfish, Ictalurus punctatus Fish & Shellfish Immunology 2010
Identification and characterization of matrix metalloproteinase-13 sequence structure and expression during embryogenesis and infection in channel catfish (Ictalurus punctatus) Developmental and Comparative Immunology 2010
GENERATION AND ANALYSIS OF ESTs FROM THE GRASS CARP, CTENOPHARYNGODON IDELLUS Animal Biotechnology, 2010
Molecular characterization of complement factor I reveals constitutive expression in channel catfish Fish & Shellfish Immunology 2009
NOD-like subfamily of the nucleotide-binding domain and leucine-rich repeat containing family receptors and their expression in channel catfish Developmental and Comparative Immunology 2009
Transcriptomic profiling of Ichthyophthirius multifiliis reveals polyadenylation of the large subunit ribosomal RNA Comparative Biochemistry and Physiology, Part D 2009
Microarray analysis of gene expression in the blue catfish liver reveals early activation of the MHC class I pathway after infection with Edwardsiella ictaluri Molecular Immunology 2008
The warm temperature acclimation protein Wap65 as an immune response gene: Its duplicates are differentially regulated by temperature and bacterial infections Molecular Immunology 2008
Characterization, polymorphism assessment, and database construction for microsatellites from BAC end sequences of channel catfish (Ictalurus punctatus): A resource for integration of linkage and physical maps Aquaculture 2008
The two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with Edwardsiella ictaluri Developmental and Comparative Immunology 2008
Assessing genetic diversity of populations of topmouth culter (Culter alburnus) in China using AFLP markers Biochemical Systematics and Ecology 2007
Generation and analysis of ESTs from the eastern oyster, Crassostrea virginica Gmelin and identification of microsatellite and SNP markers BMC Genomics 2007
Repeat structure of the catfish genome: a genomic and transcriptomic assessment of Tc1-like transposon elements in channel catfish (Ictalurus punctatus) Genetica 2007
Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs BMC Genomics 2007
Structural characterisation and expression analysis of Toll-like receptor 2 gene from catfish Fish and Shellfish Immunology 2007
A BAC-based physical map of the channel catfish genome Genomics 2007
Evolution of CC chemokines in teleost fish: a case study in gene duplication and implications for immune diversity Immunogenetics 2007
Divergent Toll-like receptors in catfish (Ictalurus punctatus): TLR5S, TLR20, TLR21 Fish and Shellfish Immunology 2007
Expression analysis of the acute phase response in channel catfish (Ictalurus punctatus) after infection with a Gram-negative bacterium Developmental and Comparative Immunology 2007
A cDNA Microarray for Crassostrea virginica and C. gigas Marine Biotechnology 2007
Aquaculture Genome Technologies Book ( editied ) 2007
Characterization of a BAC Library from Channel Catfish Ictalurus punctatus : Indications of High Levels of Chromosomal Reshuffling Among Teleost Genomes Marine Biotechnology 2007
Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs BMC Genomics 2007
Characterization of a NK-lysin antimicrobial Fish and Shellfish Immunology 2006
Major Histocompatibility Complex Class IIB Allele Polymorphism and Its Association with Resistance/Susceptibility to Vibrio anguillarum in Japanese Flounder (Paralichthys olivaceus) Marine Biotechnology 2006
Transgenic Fish - Where We Are and Where Do We Go? The Israeli Journal of Aquaculture Bamidgeh 2006
Transcriptome Characterization Through the Generation and Analysis of Expressed Sequence Tags: Factors to Consider for a Successful EST Project The Israeli Journal of Aqaculture Bamidgeh 2006
Characterization of 23 CC chemokine genes and analysis of their expression in channel catfish (Ictalurus punctatus) Developmental and Comparative Immunology 2006
Combined use of 16S ribosomal DNA and automated ribosomal intergenic spacer analysis to study the bacterial community in catfish ponds Letters in Applied Microbiology 2006
The catfish liver-expressed antimicrobial peptide 2 (LEAP-2) gene is expressed in a wide range of tissues and developmentally regulated Molecular Immunology 2006
Molecular systematics of bisexual Artemia populations Aquacultural Research 2006
Catfish CC chemokines: genomic clustering, duplications, and expression after bacterial infection with Edwardsiella ictaluri Molecular Genetic Genomics 2006
Comparison of domestic and wild channel catfish (Ictalurus punctatus) populations provides no evidence for genetic impact Aquaculture 2006
Toll-like receptor 3 and TICAM genes in catfish: species-specific expression profiles following infection with Edwardsiella ictaluri Immunogenetics 2006
NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis Molecular Immunology 2006
Genomic organization, gene duplication, and expression analysis of interleukin-1B in channel catfish (Ictalurus punctatus) Molecular Immunology 2006
Channel catfish BAC-end sequences for marker development and assessment of syntenic conservation with other fish species Animal Genetics 2006
CC chemokines in zebrafish: Evidence for extensive intrachromosomal gene duplications Genomics 2006
Analysis of a catfish gene resembling interleukin-8: cDNA cloning, Developmental and Comparative Immunology 2005
Catfish hepcidin gene is expressed in a wide range of tissues Developmental and Comparative Immunology 2005
Characterization and expression analysis of bactericidal Developmental and Comparative Immunology 2005
Analysis of genetic variation in selected stocks Biochemical Systematics and Ecology 2005
Constitutive expression of three novel catfish CXC chemokines: Molecular Immunology 2005
Amplified Fragment Length Polymorphism (AFLP) ( Book Chapt. ) Stock Identification Methods 2005
Chemokines in Fish: A Rapidly Expanding Repertoire Focus on Immunology Research 2005
Isolation of SFRS3 gene and its differential expression during metamorphosis involving eye migration of Japanese flounder Paralichthys olivaceus Biochimica et Biophysica Acta 2005
In silico identification and expression analysis of 12 novel CC chemokines in catfish Immunogenetics 2005
DNA marker technologies and their applications Aquaculture 2004
Molecular cloning of proopiomelanocortin cDNA and multi-tissue General and Comparative Endocrinology 2004
Sequence analysis and expression of a CXC chemokine in resistant Developmental and Comparative Immunology 2004
Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA Comparative Biochemistry and Physiology 2004
Multiple CC chemokines in channel catfish and blue catfish as revealed by analysis of expressed sequence tags Immunogenetics 2004
Bioinformatic Mining of Type I Microsatellites from Expressed Sequence Tags of Channel Catfish (Ictalurus punctatus) Marine Biotechnology 2004
Development of Expressed Sequence Tags from Eastern Oyster (Crassostrea virginica): Lessons Learned from Previous Efforts Marine Biotechnology 2004
Development of Expressed Sequence Tags from Eastern Oyster (Crassostrea virginica): Lessons Learned from Previous Efforts Marine Biotechnology 2004
Alterations in Gene Expression in the Brain of White Catfish (Ameiurus catus) in Response to Cold Acclimation Marine Biotechnology 2004
Identification of mariner elements from house flies ( Musca domestica )and German cockroaches ( Blattella germanica ) Insect Molecular Biology 2004
Development of type I markers in channel catfish through intron sequencing Animal Genetics 2004
Assessing genetic diversity of domestic Aquaculture 2003
Dress-out and fillet yields of channel catfish, blue catfish, and their F1, F2 and backcross hybrids Aquaculture 2003
A review of catfish genomics: progress and Comparative and Functional Genomics 2003
Translational machinery of channel catfish: II. Complementary DNA and Gene 2003
An AFLP-Based Genetic Linkage Map of Channel Catfish (Ictalurus punctatus) Genetics 2003
Gene Mapping,lsolation and Genetic Improvement in Catfish ( Book Chapter ) Aquatic Genomics - Steps Toward a Great Future 2003
Genomics revolution: great - expectations 50 Years of DNA 2003
Putative SNP discovery in interspecific hybrids of catfish by comparative EST analysis Animal Genetics 2003
Molecular characterization and differential expression of the myostatin Biochimica et Biophysica 2002
Translational machinery of channel catfish: I. A transcriptomic approach Gene 2002
Expression Profile of the Channel Catfish Spleen: Analysis of Genes Involved in Immune Functions Marine Biotechnology 2002
Differential gene expression in the brain of channel catfish ( Ictalurus punctatus) in response to cold acclimation Molecular Genetic Genomics 2002
Transcriptome analysis of channel catfish (Ictalurus punctatus): initial analysis of gene expression and microsatellite-containing cDNAs in the skin Gene 2002
Biochemical Genetics of Brook Trout: Management Implications in Georgia Annual conference of the Southeastern Association of Game and Fish Agencies 2002
Microsatellite-Containing Genes from the Channel Catfish Biochemical and Biophysical Research Communications 2001
Multiple isoforms and an unusual cathodic isoform of creatine kinase from Gene 2001
Channel Catfish Follicle-Stimulating Hormone and Luteinizing Hormone: Complementary DNA Cloning and Expression During Ovulation Marine Biotechnology 2001
Effects of Insert Size on Transposition Efficiency of the Sleeping Beauty Transposon in Mouse Cells Marine Biotechnology 2001
Detection ofYersinia ruckeri in rainbow trout blood by use of the polymerase chain reaction Disease if Aquatic Organisms 2001
Transcriptome of channel catfish (lctalurus punctatus): initial analysis of genes and expression profiles of the head kidney Animal Genetics 2001
Genetic Analysis of Ozark Hellbenders Utilizing RAPD Markers Proceedings of the Annual Conference Southeast Association of Fish and Wildlife Agencies 2001
Biochemical Genetics of BrookTrout in Georgia: Management Implications Proceedings of the Annual Conference Southeastern Association of Fish and Wildlife Agencies 2001
Gene Mapping and Marker-assisted Selection in Channel Catfish (Ictalurus Punctatus) Acta Agriculturae Boreali-occidentalis Sinica 2001
Review of the Status of Aquaculture Genetics Technical Proceedings of the Conference on Aquaculture in the Third Millenium 2001
The skeletal muscle a-actin gene of channel catfish (Ictalurus Gene 2000
Transcriptome analysis of channel cat Gene 2000
Comparison of Manual Stripping and Pen Spawning for Production of Channel Catfish 3 Blue Catfish Hybrids and Aquarium Spawning of Channel Catfish North American Journal of Aquaculture 2000
Random amplified polymorphic DNA markers: Aquaculture 1999
Transcribed Dinucleotide Microsatellites and Their Biochemical and Biophysical Research communications 1999
Characterization of nonautonomous Tc1-like transposable elements of Fish Physiology and Biochemistry 1999
High levels of conservation at microsatellite loci among Ictalurid catfishes Journal of Heredity 1999
Development of Polymorphic EST Markers Suitable for Genetic Linkage Mapping of Catfish Marine Biotechnology 1999
Development of Amplified Fragment Length Polymorphism (AFLP) Markers Suitable for Genetic Linkage Mapping of Catfish Transactions of the American Fisheries Society 1999
Effects of Seasonal Variations, Thyroid and Steroid Hormones, and Carp Pituitary Extract on the Artificial Production of Channel Catfish Zctalurus punctatus X Blue Catfish I. furcatus Hybrids Journal of the World Aquaculture Society 1999
Polymorphic microsatellite markers in Ictalurus punctatus and related catfish species Molecular Ecology 1999
Inheritance and usefulness of AFLP markers in channel cat Molecular and General Genetics 1998
Characterization of an A/T-rich family of sequences from channel catfish (Ictalurus punctatus) Molecular Marine Biology and Biotechnology 1998
The Effect of the Absence or Presence of Channel Catfish Males on Induced Ovulation of Channel Catfish Females for Artificial Fertilization with Blue Catfish Sperm Progressive Fish Culturist 1998
Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2 and backcross hybrids Animal Genetics 1998
Transcriptional activities in the pituitaries of channel catfish before and after induced ovulation by injection of carp pituitary extract as revealed by expressed sequence tag analysis Journal of Molecular Endocrinology 1998
Genetic linkage and QTL mapping of ictalurid catfish Circular 1998
REVIEW OF PROGRESS IN CATFISH GENE MAPPING Proceedings of the Aquaculture Species Genome Mapping Workshop 1997
Hetero-stagger cloning: efficient and rapid cloning of PCR products Nucleic Acids Research 1996
The E5 gene product of rhesus papillomavirus is an activator of endogenous Ras and phosphatidylinositol-3'-kinase in NIH 3T3 cells Proceedings of the National Academy of Science-Biochemistry 1996
The expression levels of the human papillomavirus type 16 E7 correlate with its transforming potential Virology 1995
Incompatibility conditioned by the Mla gene in powdery mildew of barley" timing of the effect of cordycepin on hypersensitive cell death Physiology and Molecular Plant Pathology 1994
The E6 gene of human papillomavirus type 16 is sufficient for transformation of baby rat kidney cells in cotransfection with activated Ha-ras Virology 1994
Folic Acid and Pteroic Acid Related to Anemia of Cultured Channel Catfish, Ictalurus punctatus Journal of Applied Aquaculture 1993
Molecular Cloning and Analysis of Abundant and Stage-Specific mRNAs from Puccinia graminis Molecular Plant-Microbe Interactions 1993
The products of the E5, E6, or E7 open reading frames of RhPV 1 Can individually transform NIH 3T3 cells or in cotransfections with activated Ras Can transform primary rodent epithelial cells Virology 1993
Folate-Degrading Bacteria in Channel Catfish Feeds Journal of Applied Aquaculture 1991
Development of Expression Vectors for Transgenic Fish Biotechnology 1990
Functional Analysis of Elements Affecting Expression of the B-Actin Gene of Carp Molecular and Cellular Biology 1990
Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds Diseases of Aquatic Organisms 1989

126 Records Total


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