Video S1 Formation of Xf cell aggregates in Zn-amended medium over time. Re-injection of Xf in a microfluidic chamber (0.1 µl min-1, overnight) after incubation for 7 days in PD2 amended with 400 µM ZnSO4. Flow rate of the medium was maintained at 0.25 µl min-1 throughout the experiment. Scale bar represents 20 µm.
S2A
S2B
Video S2 Shear force resistance of Xf cell aggregates formed in PD2 and PD2 amended with Zn after extensive re-injection of a microfluidic chamber. Note how the aggregates formed in PD2 (A) are washed away by the medium flow while the aggregates formed in Zn-amended PD2 (B) remains static. The media flow rate is 0.25 µl min-1 for both channels. Scale bar represents 20 µm.