Figure 1. Seedlings with RCD annotation.

            Alabama is the second largest producer of seedlings in the country, growing more than 237 million seedlings per year in 1997. The number of seedlings per nursery varies between 2.5 and 6 million per year. An important characteristic of a seedling is the root collar diameter (RCD) as shown in Figure 1 (right). It is a measure of the seedling’s survivability potential and also the parameter on which the seedling price is based.  Nurseries measure the RCD of thousands of seedlings each year to obtain a statistical measure of the crop value in the field.

            Traditionally, the RCD is measured by hand with a slide micrometer, a tedious and time consuming task. AAES researchers in the Auburn University Department of Biosystems Engineering developed an alternative that measures the RCD automatically. The user can place a seedling on a measurement table and at the push of a button the RCD is measured and stored in a spread sheet.

            The principle of measuring the seedling diameter is shown in Figure 2. A single diverging infrared light beam is used in combination with two optical sensors.  When a seedling is blocking the light beams, the signals emerge. The velocity of the seedling is obtained from the time difference between interruption of layer 1 and 2, respectively. The RCD follows from multiplying the velocity by the total time a seedling blocks either light layer. The constant b is the distance between the light layers, which is a constant as long as the distance from the seedling passage path to the sensors is constant. The formula used to determine the RCD is:
where:

D: Diameter oftheobject (RCD) (m)

b: Distance between centers of optical sensors (m)

: Flank separation signal (s)

: Pulse width of a single sensor signal (s)

The timing signals are measured using a dedicated board and automatically put in a spread sheet.

            The first prototype of the sensor consisted of an arrangement where the user manually slid the seedling along a set of sensors (Figure 3). This concept proved to be cumbersome, because a human being is not capable of maintaining a constant velocity during measurement. The assumption that the distance between the seedling and the sensors is constant does not apply here; it depends on the diameter itself. Another fact is that the orientation of the seedling is often not exactly vertical, which means that the measured diameter is larger than the true RCD.

            The manual sliding device was calibrated with drill bits of diameters [shown in millimeters (mm) and also inches]: 3.17 mm (1/8 inch), 3.57 mm (9/64 inch), 3.97 mm (5/32 inch), 4.34 mm (11/64 inch), 4.74 mm (3/16 inch), 5.12 mm (13/64 inch), 5.53 mm (7/32 inch), 5.92 mm (15/64 inch), 6.33 mm (1/4 inch), 6.69 mm (17/64 inch), 7.91 mm (5/16 inch), and 9.46 mm (3/8 inch). The diameters of the drill bits were measured with a slide-micrometer with an accuracy of 1/100 mm. The calibration curve is shown in Figure 4. The standard deviation among 10 measurements of the smallest object (3.17 mm) was 0.01 mm (0.3%) and increased linearly to 0.07 mm (0.7%) for the largest diameter (9.46 mm).

            One problem with the manual sliding device is that the distance between the sliding path and the center of the seedling is not constant. This will introduce errors because the optical beam used is not parallel. This error can be virtually eliminated by placing the sensors in a balance configuration as shown in Figure 5. A dual opposing set of sensors is placed such that errors due to lateral movement of the seedling are compensated by the opposing sensor set.

            To eliminate the errors introduced by the skill of the user, a new concept was developed where the seedling is placed on a table and the sensors themselves are moved. Proper alignment of the seedling is aided by projecting a laser line on the measurement table. Also, the balanced configuration shown in Figure 5 was incorporated in this new design. To move the sensors with respect to the seedling, the device uses a counterweighted bridge principle as shown in Figure 6. The seedling is the horizontal wood-textured bar and the sensors are the white dots in the black square. In idle, the sensors are below the surface of the table. When released, the counterweight on the left drops freely and the sensors move upward under a constant acceleration. Because of this acceleration a new formula was developed to obtain the RCD as follows:


where:

: Diameter of the object (RCD) (m)

b :Distance between centers of optical sensors (m)

: Flank separation signal (s)

: Pulse width of a single sensor signal (s)

a: Acceleration of the sensors (ms-2)

            This equation naturally transforms into the zero-acceleration version when a is set to zero. The counter weighted RCD measurement system was calibrated with the same set of drill bits used in the manual sliding device. The calibration curve is shown in Figure 7. The non-linearity of the curve is caused by the acceleration of the sensors but the reproducibility of the arrangement was dramatically improved over the manual sliding device. The standard deviation among ten measurements of the smallest object (3.17 mm) was 0.005 mm (0.15%) and increased linearly to 0.02 mm (0.2%) for the largest (9.46 mm).

            The sensor as developed here could have a great potential for nurseries to reduce a tedious task inexpensively at a total hardware cost of around $600.


Grift is Assistant Professor of Biosystems Engineering.

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